human prostate tissue extract  (Santa Cruz Biotechnology)

Journal:

Article Title: Expression of colony-stimulating factor 1 receptor during prostate development and prostate cancer progression

doi: 10.1073/pnas.222537099

Figure Lengend Snippet: Expression analyses of CSF-1R in human prostate cancer cell lines. (A) RT-PCR analysis. The PCR products at representative cycles were shown: CSF-1R, CSF-1, and prostate-specific antigen, cycle 40 and β-actin, cycle 25. The primers sequences are listed in Materials and Methods. (B) Immunoblotting analysis. Protein lysates of each 2 × 105 cells were subjected to SDS gel electrophoresis and polyclonal rabbit anti-human CSF-1R antibodies (1:250 dilution) were used for this analysis, as described in Materials and Methods. Human breast cancer cell line BT-20; human prostate cancer cell lines PC3, DU-145, LNCap, and LAPC4, and normal basaloid PrEC prostate cells were used in these analyses. PC-3 infected with human CSF-1R expressing retrovirus was used as a control.

Article Snippet: Human prostate cancer tissue specimens were examined by IHC for CSF-1R expression with rabbit anti-CSF-1R peptide antiserum (Santa Cruz Biotechnology).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, SDS-Gel, Electrophoresis, Infection

Journal:

Article Title: Expression of colony-stimulating factor 1 receptor during prostate development and prostate cancer progression

doi: 10.1073/pnas.222537099

Figure Lengend Snippet: CSF-1R immunostaining in human primary prostate cancer with polyclonal rabbit anti-human CSF-1R antibodies (Santa Cruz Biotechnology) based on the ABC method (DAKO) or the EnVision+ System (DAKO). (A) Stronger staining in tumor cells was seen compared with adjacent normal glands. (B) Control IgG staining on a serial section. (C) Specific immunostaining was observed in the cytoplasm of PIN as well as malignant epithelial tumor cells. (D) Macrophage origin defined by CD68 staining (arrow: luminal macrophage in prostatic gland) using the double-staining method as described in Materials and Methods. CD68 staining used DAB (brown) and CSF-1R staining used Fast red (red purple) as substrates. (E) CSF-1R staining was detected in brush border of placental syncytiotrophoblasts (arrows) and villous macrophages (arrowheads). (F) After preincubation with the immunizing peptide, the staining for CSF-1R was blocked.

Article Snippet: Human prostate cancer tissue specimens were examined by IHC for CSF-1R expression with rabbit anti-CSF-1R peptide antiserum (Santa Cruz Biotechnology).

Techniques: Immunostaining, Staining, Double Staining

Journal:

Article Title: Expression of colony-stimulating factor 1 receptor during prostate development and prostate cancer progression

doi: 10.1073/pnas.222537099

Figure Lengend Snippet: Immunohistochemical staining distribution of prostate by anti-CSF1R antibody on tissue microarrays. Two hundred fifteen prostatectomy cases encompassing 1,109 informative tissue spots on three TMAs were used for analysis grading on a 0–2 scale (0 = negative, 1 = weak, and 2 = strong staining). Immunostaining conditions were as described in Materials and Methods. NL, normal glands; BPH, benign prostatic hyperplasia; PIN, prostatic intraepithelial neoplasia; PCA, prostate cancer. Tissue spot histology and grading were confirmed on hematoxylin/eosin slides.

Article Snippet: Human prostate cancer tissue specimens were examined by IHC for CSF-1R expression with rabbit anti-CSF-1R peptide antiserum (Santa Cruz Biotechnology).

Techniques: Immunohistochemical staining, Staining, Immunostaining

Journal:

Article Title: Expression of colony-stimulating factor 1 receptor during prostate development and prostate cancer progression

doi: 10.1073/pnas.222537099

Figure Lengend Snippet: Immunohistochemical analysis of CSF-1R and activated CSF-1R expression in metastatic prostate cancer. Tissue sections from primary and metastatic prostate cancer (aorta) were stained with polyclonal rabbit anti-CSF-1R (Santa Cruz Biotechnology) antibody using the EnVision+ System (DAKO) and polyclonal rabbit anti-PY723 CSF-1R antibody using both the EnVision+ system and the tyramide signal amplification system (NEN). Coincidence of CSF-1R with activated CSF-1R in some sections was confirmed by serial sections. The specificity of anti-PY723 CSF-1R antibody was confirmed after preincubation with immunizing PY723 peptide (10 μM) blocked reactivity (39).

Article Snippet: Human prostate cancer tissue specimens were examined by IHC for CSF-1R expression with rabbit anti-CSF-1R peptide antiserum (Santa Cruz Biotechnology).

Techniques: Immunohistochemical staining, Expressing, Staining, Amplification